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KMID : 1007520090180051253
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Food Science and Biotechnology
2009 Volume.18 No. 5 p.1253 ~ p.1257
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Production of Exo-polysaccharide from Submerged Culture of Grifola frondosa and Its Antioxidant Activity
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Lee Keyong-Ho
Yoon Won-Ho
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Abstract
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Exo-polysaccharide isolated from the culture of Grifola frondosa was modified by sodium periodate (NaIO4) and sodium chlorite (NaClO2) to delete polysaccharide part and phenolic compound, respectively, and was investigated what effect has each part of exo-polysaccharide against 2,2’-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced oxidative stress in porcine kidney epithelial cells (LLC-PK1). Oxidative stress on LLC-PK1 cell was measured by cell viability, lipid peroxidation, superoxide dismutase (SOD), and glutathione peroxidase (GSH-px) activity. Exposure of LLC-PK1 cells to 1 mM AAPH for 24 hr resulted in significant decrease in cell viability, SOD, and GSH-px action, and significant increase in lipid peroxidation. The treatment of exo-polysaccharide and NaIO4 modified sample protected LLC-PK1 cells from AAPHinduced cell damage such as cell viability, lipid peroxidation, SOD, and GSH-px activity in a dose dependant manner (10, 100, and 500 μg/mL). However, the treatment of NaClO2 modified sample did not affect for cell viability, lipid peroxidation, SOD, and GSH-px activity. The antioxidant activity of exo-polysaccharide was significantly decreased on AAPH-induced LLC-PK1 cell system when phenolic compound was deleted. The antioxidant activity was significantly correlated with the content of phenolic compound of exo-polysaccharide.
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KEYWORD
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Grifola frondosa, antioxidant, 2, 2’-azobis(2-amidino propane) dihydrochloride (AAPH), porcine kidney epithelial cells (LLC-PK1)
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